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The harvested cultures were clarified by centrifugation, and the supernatants were inoculated onto fresh MDCK cells as described for the initial inoculation; up to 3 additional passages were performed for samples that did not show evidence of influenza virus by hemagglutination or RT-PCR.
These animals are probably responsible for the initial inoculation of the immediate environment.
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Measurements were made from the initial inoculation until 18.5 days.
Five days after inoculation, both Ph. autumnale (CAWBG26) and Planktothrix sp. (CAWBG59) had filament growth extending from the initial inoculation site across the walls of the culture bottles.
For all analyses, the initial inoculation of Synechococcus cells was at an initial OD730 of about 0.075 in BG-11 media [ 22] and cultured at 30 °C and 40 μmol⋅m−2⋅s−1.
The inoculated roots were harvested at 3 hrs, 27 hrs, and 51 hrs after the initial inoculation for RNA extraction.
Serum collected from the patient day 20 after the initial inoculation was tested for vaccinia virus specific immunoglobulin (Ig) G response by enzyme-linked immunosorbent assay (ELISA) as described (9, 10 ).
The initial inoculation density was maintained same for all the three systems in both the studies.
These interventions, taken together, are called "the initial inoculation" here.
Rechallenge of the group B cats occurred 671 days after the initial inoculation of M. haemofelis.
The cultured biomass underwent RNA extraction four weeks after the initial inoculation.
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