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Stimulation was performed for the indicated time with 1.0 mM of freshly prepared CaCl2 for the indicated time.
HEK293T WT or Nedp1 −/− cells were treated with 800 μmol/L H2O2 for the indicated time.
Cell entry was initiated by raising the incubation temperature to 37°C for the indicated time.
The cells were incubated under normoxia at 37°C for the indicated time.
After infection, the cells were incubated in fresh media containing 0.06 mM or 2 mM calcium for the indicated time.
Serum-starved cells or cells growing in 10% serum were treated with EGF for the indicated time.
Fluorescence recovery was recorded for the indicated time.
The cells were infected with VSV for the indicated time.
To generate osteoclasts, the BMMs were treated with RANKL (50 ng/mL) for the indicated time.
Then, safingol at 10 μ was added to cells for the indicated time.
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Monocytes were incubated with IC for the indicated time-points.
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