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The infected cells were then incubated in gentamicin media for the indicated length of time and assayed as described below.
Briefly, mid-log cell cultures were grown on appropriate medium, and treated or not treated with 0.5 M KCl for the indicated length of time.
In brief, cells were treated with or without bortezomib for the indicated length of time.
Approximately 2 × 10/ml MDA-MB-231 cells were transfected with ANT1 and cultured for the indicated length of time.
Following incubation with POEE for the indicated length of time, cytotoxicity was determined using the Vybrant cytotoxicity assay kit (Sigma Aldrich, MO, USA), following the manufacturer's procedure.
mDF cultured for 2 days in 0.2% FBS were infected with Ha-Ras expressing and control lenti-particles for the indicated length of time.
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(A and D) Confocal microscopic analysis of stable shCTRL- and shTrak1-transfected HeLa cells after starvation with HBSS (A) or exposure to protein synthesis inhibitor CHX (D) for the indicated lengths of time.
The cells were then harvested, resuspended at 107cells/ml in prewarmed EMEM supplemented with 5% FCS, and stimulated by adding 1 µg/ml DNP-HSA for the indicated lengths of time.
To investigate whether ubiquitinated FcεRI subunits could interact with UIM-containing proteins, lysates obtained from RBL cells unstimulated and stimulated for the indicated lengths of time were subjected to immunoprecipitation with an anti-IgE mAb, separated by SDS-PAGE, and analyzed by immunoblotting with anti-Eps15 and anti-Hrs Abs (Figure 6A).
Cells were serum-starved over night and exposed to agonists for the indicated lengths of time.
Briefly, cells were grown to 70% confluence in 96-well culture plates and were exposed to fA β1 40, pinocembrin, or both for the indicated lengths of time.
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