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Here, we demonstrate diatom shells functionalized with gold nanoparticles for the harvesting and detection of biological analytes (bovine serum albumin BSA) and chemical pollutants (mineral oil) in low abundance ranges, for applications in bioengineering, medicine, safety, and pollution monitoring.
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These results open new directions for light harvesting and detection devices, optical modulators, thermal emitters, and even visual design [1-4].
Callus was harvested from fracture gaps for microbial culture and detection of bacterial 16S RNA.
Samples were placed in sterilized PBS buffer, homogenized and centrifuged to harvest supernatants for virus re-isolation and detection of H-PRRSV using QPCR.
Six weeks after primary immunization, mice were euthanized and the sera were harvested for the detection of antibodies against PRRSV and splenocytes were isolated as described previously [ 25] for IFN- γ assay and lymphocyte-proliferation assay.
After 7 days the leaf tips were cut off and the untreated base of the leaves were harvested for the detection of endophytic colonization with B. bassiana by microscopy and PCR.
Bone marrow and splenocyte cells were harvested for the detection of anti-hFIX secreting cells (ACS) using a B-cell ELISpot.
Embryos were harvested for the detection of Oct4-positive cells with an anti-Oct4 mouse monoclonal antibody (Santa Cruz, CA, USA).
The cells were incubated with fAβ (500 nmol/L) for 18 h and were harvested for detection of intracellular Aβ levels by Western blotting analysis.
Magnetoacoustic detection is a new method for the noninvasive, early detection of cancer.
The cells were incubated with fAβ (500 nmol/L) for an additional 18 h and were harvested for detection of intracellular Aβ levels by Western blotting analysis.
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