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We thank the Duke microarray facility (a Comprehensive Cancer Institute and IGSP shared resource facility) for the technical support and feedback on the generation of the microarray data.
We thank the Duke Microarray Core facility (a Duke NCI Comprehensive Cancer Institute and a Duke Institute for Genome Sciences and Policy shared resource facility) for the technical support, microarray data management and feedback on the generation of the microarray data reported in this manuscript.
The generation of the microarray dataset has been previously described.
For the generation of the PCT-TC signature, microarray-based global gene expression analysis was performed on 27 individual RNA samples composed of 17 solid mouse PCTs and 10 tissue cultured PCT cell lines using Affymetrix U74Av2 microarray chips.
Whereas full-length cDNA microarrays were the first choice for the generation of microarray platforms, oligonucleotide-based chips are gradually gaining importance due to the reduction of manipulation steps and their ability to differentiate similar members of gene families [ 23].
In hybridization-based resequencing, the reference is necessary for the generation of microarray probes and for signal normalization.
Routine performance assessments using reference samples are highly recommended as a best practice for the generation of high quality microarray data [ 8].
Tissue microarrays served as "biopsy procedure" and were compared with 454 whole tissue sections obtained from the same paraffin blocks used for the generation of tissue microarrays.
We thank H. Liang and Shawn Smith for help in the generation of microarray data.
Moreover, oligonucleotides allow for the generation of microarrays for any organism for which the genome has been sequenced.
Methods for the generation of microarrays were successfully established when using antigens derived from membrane-based selection.
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