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For the full alignment and Bayesian Ka/Ks ratios obtained from the models, see Figure S3.
For the full alignment of 237,882 16S rRNAs, the supports required just one hour.
Both RSS and CSS are mostly found in the conserved regions of RIFIN proteins (see Additional file 4, rifins_high.html, for the full alignment with stringent cut-offs).
We used the alignment shown in Figure 1 to construct a phylogeny of bacteriorhodopsins (see Additional file 1 for the full alignment).
The complex model of substitution including gamma distribution and invariant sites (GTR+Γ+I) was selected for the full alignment by the hierarchical likelihood ratio test (LRT) as well as the Akaike information criterion (AIC).
Maximum likelihood phylogenetic analyses were performed as described above for the full alignment for each strategy of long-branch exclusion on each of three partition sizes of interest (full alignment, A136665, A133065, as discussed in Results).
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Results of significance testing for topology comparisons of the full alignment versus the three other largest data partitions.
Pairwise alignments of zfP2X4.1 and either hP2X4 or DdP2XA were prepared from the full alignment for use in the program Modeller [20].
YAHA now takes each potential alignment from above, and completes the calculation of the full alignment.
The best model of the amino acid substitution process was chosen from among 80 possible models using the program ProtTest 1.3 [ 102] for both the full alignment and the gap-free alignment.
For both the full alignment and the gap-free alignment, the empirically determined JTT substitution matrix [ 103] outperformed other substitution matrices, and incorporating rate variation among sites significantly improved the model (gamma distribution of rate variation among sites, α = 1.2).
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CEO of Professional Science Editing for Scientists @ prosciediting.com