Sentence examples for for the final construct from inspiring English sources

To optimize efficiency of the restriction-ligation for the final construct containing 11 transcription units (cL2-13*), a variation of this protocol was used as follows.

For generating TARGATT constructs, the PacI fragment from the final construct was subcloned into a PacI digested pBT346.3 plasmid (Applied Stem Cells).

The quality of the final construct was confirmed by sequencing.

The final construct coded for a cleavable (enterokinase) N-terminal fusion with a maltose binding protein (MBP) and a non-cleavable C-terminal 10×His affinity tag.

The fragments were then ligated with the expression vector giving the final construct pET28b-WXyn43 for expression of the gene encoding the native protein with an N-terminal LE-6 × His-sequence.

The final construct was used for functional expression.

The final construct, pilF 52b, codes for the TtPilF protein followed by a thrombin cleavage site and 6×histidine tag at the C-terminus.

The cDNA sequence encoding CgChoA was cloned into the expression vector pQE-30 such that the final construct pCgChoA coded for an N-terminal His-tag MRGSHHHHHHGSAC fused to CgChoA.

This method is therefore as efficient as currently used recombination-based cloning technologies but yields recombinant plasmids that do not contain unwanted sequences in the final construct, thus providing precision for this fundamental process of genetic manipulation.

Three of the authors personally supervised the administration of the questionnaire at their respective centres having also contributed to the final construct of the questionnaire for comprehension and content validity.

The final construct designated pYEX- Lca-KPVQ was transformed for functional expression into the ole1 elo1 yeast.