The n−1/2 decays reflect the error in the estimation of normalization factors.
The removal of variation can be understood as additive errors in the estimation of normalization factors.
In general, it is a matter of comparison between the magnitude of the error in the estimation of normalization factors and the amount of biological variation.
This explains, at least partially, the lack of reproducibility found in transcriptomics studies, especially for the detection of changes in gene expression of small-to-medium magnitude (up to 2-fold), because variation of this size is more likely to be distorted by errors in the estimation of normalization factors.
To identify bands which were not affected by the treatment for the estimation of the normalization factor, we calculated "uncorrected induction factors" (UIF) for each pair of bands in corresponding lanes as the ratio of areas of peaks corresponding to matched bands.
The model space-binary tree search (MS-BTS) algorithm was integrated to reduce the computational load in the search stage for the estimation of self-normalization warps.
Chromosomes X and Y BAC clones were omitted during the estimation of the normalization function.
We replicated DM sites between monocytes and PB using data set C, while matched 450K and bisulfite pyrosequencing (BPS) data from data set D were used to check the performance of selected normalization methods for the estimation of the percentage methylation.
A new method for the estimation of the effective anisotropic relaxation behavior based on higher order normalization schemes is introduced.
After segmentation, the segmented grey matter images were modulated by multiplication of the Jacobian determinant of the spatial normalization function, so as to allow for the estimation of volumetric differences between groups [25].
The estimation of the self-normalization warp,, for speaker, is done in a manner similar to offline VTLN.
