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In conclusion, the pentaplex PCR assay developed in this study was able to detect four genes that are essential for the detection and differentiation of Shigella at the genus and species levels simultaneously in a single test within 4 h.
Single nucleotide polymorphisms (SNPs) are important diagnostic markers for the detection and differentiation of Bacillus anthracis.
The spectrally highly resolved limb emission spectra provide a great opportunity for the detection and differentiation of cloud types.
Molecular based assays for the detection and differentiation of B. thermosphacta and B. campestris were developed and validated.
The assay was compared with a multiplex RT-PCR developed in the initial study for the detection and differentiation of the three viruses and host 18S rRNA.
Several analyte specific reagents (ASRs) are available for the detection and differentiation of HSV-1, HSV-2, and VZV in clinical specimens.
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Cross-sectional imaging, especially MRI, can play an important role in the detection and differentiation of these conditions, although biopsy is often needed for more specific histopathological diagnosis.
Finally, a one-step duplex PCR technique in a single tube for the rapid detection and differentiation of the F. necrophorum subspecies was developed.
In this study, a one-step multiplex reverse-transcriptase quantitative PCR (one-step mRT-qPCR) was developed for the simultaneous detection and differentiation of SBV, AKAV and AINV.
In particular, methods for the simultaneous detection and differentiation of H2S and thiols would provide new insight into these multifaceted biological interactions.
In this study, a one-step mRT-qPCR was developed for the simultaneous detection and differentiation of Schmallenberg virus (SBV), Akabane virus (AKAV) and Aino virus (AINV).
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