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One of the primary requirements for the design of the microarray was that it should be applicable for transcriptome analysis across a range of Brassica species.
The first is based on the annotation provided by the manufacturer, while the second is an in-house performed probe sequence-based annotation. 1. GenBank accession numbers that were used for the design of the microarray probes were used for querying the Mus musculus Unigene (UG) database build #151.
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The arnA transcript was not addressed in the microarray analysis, since only probes for protein-coding genes were used in the design of the microarray [ 34].
CS was responsible for the design of the study, participated in the microarray experiments, analysed the microarray data and drafted the manuscript.
AJK, initiated and contributed to the design of the oligonucleotides used for the microarray, setup, designed and carried out (part of) the microarray studies, participated in the MLST-, MLVA -and microarray data analysis and wrote the manuscript.
We present a novel three-step approach for the design of high-throughput microarray assays for HLA typing.
We previously described the design of a microarray for expression analysis of 137 C. elegans ncRNAs [ 14].
Sequencing of the chicken genome has provided the crucial information for the design of a comprehensive chicken transcriptome microarray.
The design of three microarray experiments with number of animal replicates for each developmental stage is presented in Table 1.
The design of a microarray and establishment of a reference transcriptome will be useful for large-scale gene expression studies.
We would like to thank Scott Tighe and Tim Hunter for their contribution to the experimental design of the microarray experiments.
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