Three Rhesus (Macaca macutum) macaques without any treatment or vaccination, 4 to 8 years old, (3 5 kg weight) were used for collection and isolation of T-cells from blood.
Study site, collection and isolation of fungal endophyte strains.
In this paper, we describe our protocol for the collection and long-distance transport of orchid root samples from Madagascar to labs in the United Kingdom (Kew) and the United States Illinoiss) leading to isolation and provisional identification of the endophytes.
Confirmation of the diagnosis relies on good practices for specimen collection, laboratory culture, and isolation of B. pseudomallei.
In addition multiplex I (See Table 1) included a primer/probe set for detection of human β-globulin DNA to check for adequate sample collection and preservation and isolation of DNA of sufficient quality.
JH performed the collection and processing of human material (including RNA isolation).
However, RNA expression profiles have been observed to change over time in such fractionated samples [4], [8] thereby implying the need for stabilization of RNA between sample collection and isolation to maintain the expression profile of blood cells.
We thank Sofia Vargas and Gema Robledo for their invaluable contribution in the collection, isolation, and storage of the DNA samples.
The fish remained in these tanks during the period between collection of behavioral data and isolation of tissues for microarray analysis (one week).
MTN participated in sample collection and isolation and characterization of bacteria.
