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Overall, the present hyphenation method is highly efficient and reliable, and hence suitable for the characterization and determination of the flavonoids of DB ethnomedicine.
In the present study, a method coupling ultra-performance liquid chromatography (UPLC) with photodiode array detection (PAD) and electrospray ionization mass spectrometry (ESI-MS) was developed for the characterization and determination of six flavonoids in DB medicine.
The results of this study showed that PCR-RFLP and RAPD-PCR, despite some limitations, are simple and powerful tools for the characterization and determination of Leishmania species polymorphisms.
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Therefore, a method for the simultaneous characterization and determination of the major active components in herbal medicines in general and DB in particular is still a top priority for accurate quality evaluation.
Alternatively, the capabilities of the single particle detection by ICP-MS (SD-ICP-MS) for the selective identification, characterization and determination of engineered nanoparticles will be also discussed.
An ultra performance liquid chromatography (UPLC) coupled with photodiode array detection (PAD) and electrospray ionization mass spectrometry (ESI-MS) method was developed for characterization and determination of six flavonoids in DB.
The T2 transgenic homozygous lines were chosen for further analyses, including phenotype characterization and determination of expression levels of Glyma11g13220 and potential downstream genes (Additional file 3).
Hence, an accurate geologic characterization of the sequestration site and determination of engineering properties are important issues for the reliability of model predictions.
Subsamples of substrate from each pot were retained for characterization and for determination of initial HS levels.
Moreover, a detailed UV vis spectroscopy was carried out in a wide pH range for characterization and pKa determination of HMNA.
Characterization of temporal expression patterns for the NF-κB network and determination of those genes under monophasic- or oscillatory control has not been experimentally addressed.
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