However, deletions disrupting 28aa periodicity did not significantly affect self-assembly of myosin fragments but altered the pattern of axial repeats in the paracrystals formed by these fragment [7] Thus, charge periodicity may not be the principal driving force for myosin assembly but is important for the assembly of molecules with proper axial staggering [7].
The protein encoded by SMA2 is required to produce the bending force necessary for the proper assembly of the prospore membrane during sporulation [ 42, 43], while SPO77 is required for spore wall formation during sporulation [ 42, 44, 45].
It is generally believed that LMP2 is a critical component for proteasome activity in that LMP2 is essential for the proper assembly of the immunoproteasome.
In mouse oocytes, Nedd1 has recently been shown to be essential for the proper assembly of the acentrosomal Meiosis I spindle, supporting the idea that Nedd1 has roles that do not require its localisation to centrosomes (Ma et al., 2010).
NEDD8 covalently binds to pVHL and modifies it to allow for the proper assembly of the fibronectin matrix, but failure to bind does not affect the ability of pVHL to ubiquinate HIF [ 31].
Cut sections are ticketed to ensure proper sizing and shading during the assembly of the garment.
Both BSD2 and RAF1 have been identified as rbcL assembly factors that are necessary for the proper assembly of Rubisco within the BS chloroplasts of C4 plants [ 27, 62].
These glycoproteins are essential for the proper assembly of functional flagella, and consequently bacterial motility, making the pseudaminic acid biosynthesis pathway a potentially important virulence target.
Because lysine glycosylation within the collagen domain has been shown to be important for the proper assembly of HMW oligomeric forms of Adiponectin [58], fish and mammalian Otolin may differ in their oligomeric structures due to differences in the extent of posttranslational modifications.
In mammalian cells, a peripheral RER membrane protein Sec16A localized at the ERES 22, 23 is needed at a stoichiometric level for the proper assembly of ERES 22. Sec16A interacts with COPII components such as Sec13 and Sec12 24– 26 and likely organizes the COPII proteins at the ERES.
