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This functional fingerprint was also observed for the ancestral construct (Figure 4B).
An identical behavior was observed for the ancestral construct (Figure 4D).
The same pattern was observed for the ancestral construct: large inward currents in DVF solution, relative stable or only slowly decaying currents with Ba2+ as charge carrier, and rapidly inactivating current when the charge carrier changed to Ca2+ (Figure 5C).
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Moreover, NF-κB activation was significantly higher in the derived 370A+375H construct than in the ancestral 370V+375H construct (Figure 3).
The typical response to changes in the extracellular solution for the ancestral and the derived construct are shown in Figure 5. Large currents were observed in ramp protocols at −100mV in DVF solution.
The graph-based reduction approach constructs the optimal model (GTR8C in Table 8) for the Ancestral Repeats dataset in 12 iterations (first column).
Jeff Mumm for the NTR construct.
Dr. E. De Robertis for the IGFR construct.
Based on a published cell-metabolic template for the ancestral E. coli strain comprising over 2000 reactions (Yoon et al., 2012), we first constructed three separate cell models for the phenotypes A, SS and FS, respectively.
For the construct is you.
Although such an actual ancestral genome is unknown, we can approximate its gene content using the inferred ancestral genome constructed by Gordon et al. (2009).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com