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The model of evolution for the ML analysis was determined using ModelTest 3.7 in Paup.

Relative quantity for qPCR analysis was determined using ΔΔCT method.

Denaturing acetonitrile gradient profiles and melting temperatures for analysis were determined using Wavemaker™ 4.1 software (Transgenomics).

Statistical significance for the experiment was determined using a one-way analysis of variance (ANOVA) test.

The multivariate analysis of independent prognostic factors for CCRT response was determined using logistic regression analysis.

Relative band density for Western blotting was determined using ImageJ gel analysis software.

The temporal position of the K point was determined using all 12 leads for this analysis.

For gene chip analysis, the quality of RNA was determined using the Agilent Bioanalyzer (Agilent Technologies).

Cut-off for abnormal body temperature was determined using ROC analysis.

The lentivirus titer was determined using fluorescence-activated cell sorter (FACS) analysis for GFP expression (BD Biosciences).

The secondary structure was determined using CDNN 2.1, software for protein secondary structure analysis.

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