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The genomic relationship required for the analysis was calculated using the Genabel 'R' package [ 37] and method of VanRaden [ 38], and then inverted applying the standard 'R' function.
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The inter-observer agreement for the visual analysis was calculated using Cohen's κ.
Statistical analysis was calculated using Statistical Package for Social Sciences 12.0.1 for Windows (SPSS Inc., Chicago, IL, USA).
Statistical analysis was calculated using Statistical Package for Social Sciences (SPSS) 12.0.1 for Windows (SPSS Inc., Chicago, Illinois, USA).
Statistical analysis was calculated using Stata v. 11 using Student's t test for mean comparison.
Gene-gene interaction analysis was calculated using χ2 analyses.
Genotype-phenotype interaction analysis was calculated using Fisher exact test.
Statistical analysis was calculated using Minitab.
Statistical analysis was calculated using z-scores.
P-values for upstream analysis were calculated using the Fisher's exact test.
All qRT-PCR data for RNA expression analysis were calculated using the ΔΔCT method.
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