It is also the leading supplier of equipment used for one of the fundamental processes in biotechnology, the amplification and detection of DNA by polymerase chain reaction, or P.C.R.
In this study, we demonstrate the feasibility of a loop-mediated isothermal amplification (LAMP) reaction, an isothermal nucleic acid amplification method, for the amplification and optical detection of HBV viral DNA in a 25 μl miniature reactor.
Purified genomic DNA was used for PCR amplification and detection of LoxP-flanked and excised Brca1LoxP/LoxP, p53LoxP/LoxP alleles.
Subsequently, a duplex PCR was designed for amplification and detection of nifH and nifD genes in indigenous photosynthetic bacteria.
ABI 7500 Real-Time PCR Detection System (Applied Biosystems, Foster City, CA) was applied for amplification and detection of cDNA following manufacturer recommended protocol.
Simultaneous isolation, amplification, and detection of a standard amount of phocid herpesvirus were used for internal control of inhibition (10 ).
It consists of 8 channels responsible for amplification, filtration and detection of biomedical signals.
Here, we developed a simple, sensitive and specific fluorescence method based on the combination of rolling circle amplification (RCA) and multifunctional molecular beacon-based strand-displacement amplification (MMB-SDA) for the amplification detection of let-7a miRNA.
Additional controls performed for each cDNA amplification included optimization of primer concentration, assessment of amplification efficiency and detection of possible primer dimerization through analysis of dissociation curves.
For amplification and colorimetric detection of bands, the Bio-Rad Amplified Opti-4CN Detection Kit was used.
