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Total RNA (1 μg) was used for template of first-strand cDNA synthesis using extent reverse transcription kit (PrimeScript RT reagent Kit with gDNA Eraser, TaKaRa, Japan).
Two microliters of each diluted reference DNA were used for template of the PCR reaction.
50 ng of cDNA from different tissues of potato plants was used for template of each reaction.
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Until recent past, the nature was considered as a compendium for templates of new chemical entities (NCEs).
Obama will try to understand how they processed complex challenges and look for templates of analysis, decision and action that might be relevant to his own life and new daunting responsibilities.
PCR products for templates of sequence were purified using PCR product presequencing kit (Amersham Life Science, Piscataway, NJ, USA).
To overcome the challenge of probe design for templates of random sequence, we adapted the universal template (UT) approach where a probe-binding sequence is appended to one of the PCR primers [ 10].
Books are available for templates of these on tutu.com.
The selective recognition capability for template BF of HM-IMs sorbents was proved to be excellent compared with structural analogs.
At first the RDB containing hand generated coordinates is scanned for a template of the given ring system.
Specifically, a value of 1 is assigned to marker.A and −1 is assigned to marker.B for the template of subclass A (template.A).A
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com