Sentence examples for for targeted modifications from inspiring English sources

Low specificity of the antibody for targeted modifications or cross-reactivity with off-target sites results in high background noise.

Since these epigenetic alterations are reversible, identification of methylated genes for targeted modifications in cancer can provide a new approach to successful drug therapies.

This provides additional flexibility in identifying suitable sites for targeted modifications, with the goal of relieving the requirement for an NGG sequence.

A series of recent publications have firmly established the notion that Drosophila researchers now have a general method to subject genes for targeted modification by homologous recombination (HR) [Science 288 (2000 20133; Genetics 157 (3) (2001 13077; Genes Dev. 16 (120022002) 1568; Genetics 161 (2002) 1125 1136].

Here we discuss the architecture of ZFNs and strategies for generating targeted modifications within the genome.

All the proteins involved in the process can be readily imported from GoSynthetic into other popular software such as Cytoscape for further investigations or to plan targeted modifications for synthetic biology and protein design.

More importantly, it provides functional groups or moieties for potential targeting modification.

These findings shed light on the regulation of protein PTMs and present a strategy for targeting two modifications with one molecule.

For targeting epigenetic modification, a catalytically inactive Cas9 variant has to be used, which still can recognize and bind the target sequence, but cannot cleave it [ 78- 80].

Further, comparative results indicate that p53 protein may also be a target for direct modification by 15d-PGJ2.

One limitation of the CRISPR system is the potential for off-target modifications, i.e., the modification of sequences similar to the intended target sequence [ 13, 36].