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Using this reasoning, we chose a grid cell size of 32 km2 for successive analyses.
Sera were then aliquoted into small volumes and stored at −20°C for successive analyses.
Only spots showing at least a two-fold change in their relative volumes were considered for successive analyses.
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For all successive analyses, cytoplasmic and nuclear staining were analysed independently.
Consequently, we eliminated the three items which were flagged and recoded the rating scale for the successive analyses into three categories: no difficulty, some/much difficulty and unable to do.
The electrodes were sufficiently durable to be used under flow conditions with no loss of reproducibility for approx. 85 successive analyses.
The presented data were obtained by five successive analyses for each sample spanning the range of the calibration curve.
Two controls (Pinot Noir and Sangiovese cultivars) were added to each set of 94 accessions for DNA extraction and successive analyses.
For the FA approach, successive analyses fitting from seven to 12 factors were carried out and the best model was chosen following the same principles as for the PC approach.
Successive analyses were run for each trait until the maximum log likelihood value was found by varying α between 0 and 1 (Christensen and Lund, 2010).
Four successive analyses were carried out for the exposure categories of starting oral contraceptives, adding new use, restarted use, and, finally, switched use.
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