The only variable that showed a significant predictive value for the probability of successfully establishing a new hESC line was having less than four cells on day two, P = 0.018 (Table 2).
It was aimed at providing the current landscape of systems metabolic engineering and suggesting directions to address future challenges towards successfully establishing processes for the bio-based production of fuels and chemicals from renewable resources.
In turn, through long battles with host, Mtb has developed a plethora of strategies to counteract the bactericidal activities of the host immunity, thereby successfully establishing a niche for long-term survival within macrophages (Jayachandran et al., 2013).
The frequency of transfers successfully establishing new generations for each replicate was quantified as the ratio of the number of transfers to Gmax (see Materials and Methods), following (Joyner-Matos et al. 2011).
The results of some studies suggest that unknown components of the soil microflora might be required for B. edulis to successfully establish a mycorrhizal relationship with the host plant.
The same type of rescue occurs for a population that starts near carrying capacity, but occupies an initial spatial size below that which is necessary for a population to successfully establish.
Although the integration property of lentivirus has abrogated it to be used in human, it is helpful for our study purpose to successfully establish stable cell lines persistently expressing siRNAs.
The pANCR low copy-number plasmid used to successfully establish infectious cDNA for WNV and JE [18] failed to produce full length infectious cDNA of DENV serotype 1 (DENV1) [10].
Attempts to successfully establish transformation protocols for some oomycetes have had, in some cases, little or no success.
First, although in situ hybridisation for HOTAIR has been successfully established for cell lines, we were not able (despite substantial efforts; data not shown) to determine the specific subset of HOTAIR-expressing cells within the bulk tumour sample.
In emergency medicine, short protocols such as focused assessment with sonography for trauma (FAST) have been successfully established for more than a decade.
