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The rate constant was obtained by fitting the data to the exponential function Ft/F0 = A1+A2 e-kt, where A1 and A2 are constants and k is the rate constant for subunit exchange.
Decrease in SITS emission intensity at 426 nm and increase in LYI emission intensity at 515 nm were recorded at every 2 min intervals and the subunit exchange rate constant (k) was calculated by curve fitting an exponential function Ft/ F0 = A1+A2 e−kt where A1 and A2 are constants and k is the rate constant for subunit exchange.
Schematic of a potential mechanism for subunit exchange in CaMKII.
10.7554/eLife.01610.004 Figure 2. Single-molecule assay for subunit exchange reveals activation-dependent subunit exchange.
An elegant mechanistic model for subunit exchange is proposed based upon structural and experimental constraints, and supported by molecular dynamics simulations.
We conclude that phosphorylation of both Thr 305 and Thr 306, in addition to Thr 286, is important for subunit exchange.
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It is worthy of mention but needs to be described in a separate paragraph and not mixed in with the discussion of the potential importance of subunit exchange for memory maintenance.
C18-micelles exhibit slightly higher kinetic stability in the presence of serum proteins at 37 °C, where the rate constant of subunit exchange is 0.20 h–1 for C18-micelles vs 0.22 h–1 for C16-micelles.
To follow kinetics of subunit exchange, light and heavy proteins were first buffer-exchanged and then equal amounts were incubated for 3 days.
The time-course of subunit exchange between FP-ABCD/CaM tetrameric complexes is affected by calcium.
(C ) The extent of subunit exchange is monitored by FRET.
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