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Pyruvate is decarboxylated by the pyruvate dehydrogenase complex (PDC) to produce acetyl-CoA, which is then used for subsequent reactions.
Moreover, enzymes used often require an inactivation step and thus cannot be recycled for subsequent reactions leading to the rise of processing costs (Kristinsson and Rasco 2000).
Roughly 400 μL of the peak activity were loaded into the storage loop within the NanoTek system and aliquots of different volumes were used for subsequent reactions.
The final reaction mixture was subjected to hydrolysis under mild alkaline conditions to give a final amide yield of 99% and the excess acylating agent remained as laurate that can be recycled for subsequent reactions.
After being washed with water and ethanol for several times and dried at 60 °C overnight, the Zn-In-4 catalyst was reused with a fresh MO aqueous solution (5 mg/l) for subsequent reactions under the identical conditions.
After completion of the reaction, as indicated by thin layer chromatography (TLC) (n-hexane/ethyl acetate 3/1), the catalyst was separated magnetically from the reaction mixture, washed with ethanol, and used for subsequent reactions after drying.
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According to this metathesis, free Cu2+ will not be available for subsequent reaction with EDTA, rather Na+ is replaced by Cu2+ ions leading to the formation of Cu2 EDTA).
Recently, Ipk1 from C. neoformans (CnIpk1) was identified as an inositol 1,3,4,5,6-pentakisphosphate 2-kinase that catalyzes the phosphorylation of IP5 to form IP6, a substrate for subsequent reaction to produce inositol pyrophosphates, such as PP-IP5/IP7.
The cystamine reduction by dithiothreitol (DTT) results in terminal thiol (–SH) groups being reduced and prepared for subsequent reaction.
Column chromatography eluting with CH2Cl2 to CH2Cl2/MeOH (95:5) afforded the desired substituted 6-phenylpyrazine-2,3-diamine, which was used for subsequent reaction without further purification.
In this way, an alcohol, enol or sulfide is converted into a good leaving group for subsequent reaction with an acceptor alcohol.
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