Sentence examples for for subsequent purification from inspiring English sources
While CFPS reactions are typically performed in batch or fed-batch reactions, a well-engineered reaction scheme may improve both the rate of protein production and the economic efficiency of protein synthesis reactions, as well as enable a more streamlined method for subsequent purification of the protein product all necessary requirements for point-of-care protein synthesis.
Ideally, radiolabelling would be achieved rapidly, under mild conditions, to a high specific activity and preferably in a simple one-pot kit-based method without the need for subsequent purification steps.
Ten days after the final injection, the immunized blood was collected by jugular puncture, and the serum was separated for subsequent purification of anti-abrin polyclonal antibodies with rProtein A Sepharose Fast Flow (GE Healthcare Bio-Sciences CorPiscatawayaway, NJ, USA).
For subsequent purification experiments, we selected a treatment consisting of sodium butyrate (1 mM) with tetracycline (1 µg/ml) for 48 hours for attempts to purify primarily the 32 kD form.
Serum obtained from the third round of bleed of animal no.13 showed the highest antibody titer and was used for subsequent purification and enrichment by antigen dependent affinity chromatography.
The supernatant was collected for subsequent purification.
Where specified, LapD was solubilized for subsequent purifications by incubating membranes in membrane buffer supplemented with 2% Triton X-100 (Sigma-Aldrich, St . Louis MO).
Extracellular localization of the recombinant proteins is highly advantageous for the subsequent purification strategies.
All enzyme constructs were designed to contain a His-tag for the subsequent purification.
In the course of this purification procedure, the fractions with the highest Pgm activities were used for the subsequent purification steps.
Only in the case of the 120 aa fragment it was possible to recover an amount of soluble protein sufficient for the subsequent purification by affinity chromatography on Gluthatione Sepharose 4B (GE Healthcare, NJ, USA).
