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Our strategy allowed to easily vary the chemical nature of the handles, which is crucial for subsequent manipulation with an STM tip.
The single-hole type dispenser successfully dispensed droplets of a solution supplied by a syringe pump on an electrode panel surface; and thus the droplets become available for subsequent manipulation on the electrode panel.
This suggests that for a PG more time was needed during the grasp closing phase as to determine more precise contact points for subsequent manipulation.
After heat denaturation and brief centrifugation (1200 g for 5 min), the supernatant containing genomic DNA was removed to a fresh tube for subsequent manipulation and storage.
Despite the advantage of having all CMV components on a single plasmid (e.g. ensuring that all CMV components are simultaneously introduced into the same cell) [ 9], the CMViva plasmid proved to not be easy for subsequent manipulation.
Once the mussels were brought to the surface, they were immediately processed onboard for subsequent manipulation of RNA or immediately stored at -80°C for long-term preservation of tissue samples.
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XbaI and XhoI sites added for subsequent manipulations are underlined.
Inspection of protein markers provides important information about the current state of the cells and data for subsequent manipulations.
The reaction shows high functional-group tolerance for both coupling partners, including halides, thus preserving the scope of the reaction for selective subsequent manipulation of the products.
Therefore, sequences for custom priming, restriction digestion, recombination, etc, can be introduced, allowing for quantification or subsequent manipulation of ligation products.
We recommend prior fixation of the femoral shaft fracture in these injuries, without attempting to reduce the femoral neck fracture initially, as it provides for more easy subsequent manipulation, reduction, and fixation of the ipsilateral femoral neck fracture.
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