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Total RNA was prepared from frozen specimens by 24 hr immersion at -80°C in RNAlater-ICE (Ambion, Austin, TX) to 'transition' solid tumors for subsequent homogenization by grinding with a liquid nitrogen-chilled mortar/pestle, followed by resuspension in Trizol (Sigma-Aldrich, E. St .Louis, Mo) and sonication to complete the tissue disruption.
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The freeze drying modifies the drug structure to obtain a brittle, fragile starting material for the subsequent homogenization step.
Aortae were then used either for myography or snap-frozen in liquid N2 before subsequent homogenization for protein analysis (see below).
The influences of Fe content and subsequent homogenization treatment on dilute Al Si alloy have been investigated.
The extreme distention of the plasma membranes permitted efficient fragmentation during subsequent homogenization.
The present work is expected to find application towards nano-inclusions and certain types of composites in addition to being the basis for subsequent non-linear homogenization schemes.
Protocols for the Maxwell® kit were followed with one exception - all samples were homogenized in 210 μL of homogenization solution, rather than the recommended 200 μL, to ensure adequate volumes of homogenate for subsequent procedures.
Use "Father" only for subsequent references.
Deamer joined Radiohead for subsequent performances.
The cDNA was used for subsequent qPCR.
tiff image format files for subsequent analysis.
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