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As gene trap system has been well-established to disrupt gene functions with selection markers/tags for subsequent functional analysis (Stanford et al., 2001), we here modified gene trap vectors and used CRISPR/Cas9 to establish a scalable tool entitled CTRL (CRISPR-mediated tagging and regulation of lncRNAs) for lncRNA tagging and expression manipulation in mammalian cells.
These genes are obvious candidates for subsequent functional characterization and for research aimed at determining their function within infection processes.
Our findings identified the possible functions of these SNP loci, and provide the basis for subsequent functional research.
These results provided a useful source to understand the evolutionary patterns of related genes and genomes and some intronless genes are good candidates for subsequent functional analyses specifically.
Early post-lesion expression of c-Fos may contribute to lesion-induced bFGF expression, which through its neurotrophic properties could be responsible for subsequent functional recovery.
The overall goal of our study is to build a quantitative, architectural model of the pancreatic ZG which will direct new hypotheses for subsequent functional analysis of this prototypic secretory granule.
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Our work introduces a fundamental framework for selection of appropriate candidate genes for the subsequent functional analysis of the OsGELP family members.
We chose for all subsequent functional studies the trkH mutant strain carrying an insertion after the 216 bp of trkH.
Since the structural integrity of the oxidized antibody is a prerequisite for the subsequent functional analysis of specific oxidized Met and Trp residues, the application of H2O2 was not further evaluated for the induction of Trp oxidation.
However, such a useful technical approach has the not negligible inconvenience of providing cellular material not exploitable for eventual subsequent functional studies.
Given the abundant plant area measures obtained with PAE, a nonlinear model is applied to generate precise growth curves which are suitable for the subsequent functional data analysis.
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