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For subsequent expression array hybridization, a minimum of 200 ng/μL final concentration of labeled RNA was used.
Further study found that expression of Scx is essential for subsequent expression of Tnmd [27].
Based on phage ELISA results, the strongest binders were isolated and used for subsequent expression and purification of POM scFv.
These domains were cloned into the pET28a vector (Novagen, His-tag on the N-terminus) for subsequent expression and purification.
A set of 11 organs from 5 wild-type and 5 heterozygous C3.Dll1tm1Gos/+ male animals was archived for subsequent expression profiling analysis [24].
These were augmented by 90 genes for which literature review suggests a potential role as breast cancer markers, resulting in 134 genes (Table 2) for subsequent expression analysis by PCR.
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Therefore, GS115 F2 was used for the subsequent expression of rhμPlg.
All samples were amplified in triplicate and the average CT values were calculated for their subsequent expression analysis.
The proper temporal expression of these myogenic transcription factors is essential for the subsequent expression of genes involved in muscle function.
The results suggest that the assembled transcripts are reliable and the designed primer pairs are suitable for the subsequent expression experiments.
The normalised read counts obtained from DESeq2 were used for all subsequent expression analyses, e.g. PCA, which were performed in R, with the exception of the differential gene expression analyses, which were performed using DESeq as it has been shown to be the most conservative of the currently available methods with the lowest false discovery rate [ 74].
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