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Aliquots were preserved in liquid nitrogen for subsequent expansion and examination.
This can be the basis for subsequent expansion and understanding the many biologically observed variations on this common theme.
Much effort was put into building a management system for capacity building, the training of the national master trainers, and the development of the basic training modules, thereby constructing a solid basis for subsequent expansion.
DOI: http://dx.doi.org/10.7554/eLife.00824.005 For example, to explore the cellular makeup of the pigmented layer of the eyecups, biopsies were taken, gently dissociated, and subcultured for subsequent expansion and microscopic analysis.
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In the case of subsequent expansion, antibody-microbead complexes were enzymatically detached from CD166+ cells by chymopapain treatment for 20 minutes at 4°C (10 U/106 cells; Sigma-Aldrich).
The inflammatory process and the accompanying immunological responses provide a microenvironment essential for malignant transformation and for the subsequent expansion of the transformed clone.
Subsequent explosions from this collapsing vacuum released the energy in this vacuum, reinvigorating the cosmic inflation and setting the scenario for the subsequent expansion of the universe.
Golitsyn also directed the negotiations with China and concluded the Treaty of Nerchinsk (ratified 1689), which set the Russo-Chinese border along the Amur River, thereby preparing the way for Russia's subsequent expansion to the Pacific Ocean.
Since the launch of Roll Back Malaria in 1998 there has been increased funding for malaria control and subsequent expansion in the coverage of malaria interventions. 1, 2 As a consequence, some areas of Africa have witnessed a marked decline in malaria transmission and disease burden.
Carbon dioxide and water vapour account for approximately 90% of the subsequent expansion of the batter, the remaining 10% being due to thermal expansion.
In this study we aimed to assess the evidence for separate clades that might be associated with different refugial origins, and whether there is any evidence for a population bottleneck and subsequent expansion (as might be related to the Last Glacial Maximum).
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CEO of Professional Science Editing for Scientists @ prosciediting.com