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Second, we had to use a vehicle that permits dissolution of CPF in high concentrations because of the need for subsequent dilution in culture medium.
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For subsequent dilutions into antibiotic-containing media (P1, P2, etc)., sequencing was performed after entry into stationary phase.
Cells overexpressing Pin1 and Fbxw7α, instead, showed a significant recovery of tumor formation, since tumors grew in seven out of nine mice, a trend that was observed also for the subsequent dilution of 150 000 cells.
The latter buffer was used for all subsequent dilutions.
This step was repeated with another centrifugation (215.000 g for 30 min) and a subsequent dilution of the pellet in 1 ml of 1% sarcosyl and 10% NaCl (1 h, 37°C) was carried out.
After treatment at pH 1.0 for 15 min and immediately after subsequent dilution in McIlVaine buffers at pH 7.0, there was no reassembly of the CtxB monomer into dimer or intermediates of higher stoichiometry (Fig. 3A, lane 4).
He offers a smart allegory for the appropriation of black culture (and subsequent dilution or clueless mishandling) by white people and a wry commentary on what happens when white people decide they know how to improve the lives of minorities (hint: bad fashion choices are involved).
Third, the subsequent post-treatment of the sample after plasma treatment including the resuspension liquid and time, the subsequent dilution, and the volume used for spreading on agar.
Tween 80- and Tween 80/ethanol-based formulations with subsequent dilution using aqueous media have been tested for paclitaxel.
Working standard solutions (100.0 μg/mL) were prepared by subsequent dilution to 25 mL with methanol.
For BiFC experiments, stable HEK293T β-arrestin-Gn clonal cell lines were first generated by zeocin selection and subsequent dilution cloning.
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