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A fraction was cytospun on microscopic slides for staining with Diff-Quick (Baxter Scientific Products, McGaw Park, IL) for subsequent differential counts.
Then, the reference transcriptome was prepared and RSEM tool was used to estimate abundance of reads for subsequent differential expression.
For this reason, after filtering genes with a RPKM mean filter, raw counts are used for the subsequent differential analysis.
Among these, 674 proteins had peptide spectrum matching (PSM) ≥ 5, and these were used for the subsequent differential expression analysis.
The other proximal and distal biopsies were placed immediately in 4% paraformaldehyde for subsequent histological evaluation.
Serum samples were collected at the time of sacrifice by aortic puncture and stored at -20° for subsequent biochemical evaluation.
We suggest that the differential psychiatric outcome within our preterm group relates to a differential incidence of neonatal adversities and a subsequent differential risk for abnormal brain development and organisation.
Later, coffee manufacturers could select a flavor benchmark for subsequent real-time evaluation on the production line.
Biological samples have also been collected as a resource for subsequent evaluation.
Three types of NHs were prepared by partial oxidation for opening the NH gates for subsequent evaluation of adsorption phenomena inside the nanopores.
Sessions were video recorded for subsequent detailed quantitative evaluation of avoidance/stress behavior responses during the 3-min auditory stimulus.
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