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More samples were fixed in Lugol solution for subsequent determination in sedimentation chambers using Utermöhl technique while others were fixed in glutaraldehyde to account for picoplankton under the epifluorescence microscope.
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Model drugs, ciprofloxacin, and diclofenac sodium, were dissolved in PBS pH 8 to generate standard calibration curves for subsequent determination of the drug entrapped in the optimised fibre.
Livers were immediately removed and frozen in liquid nitrogen for subsequent determination of transcript abundance.
The fluid was then stored in small aliquots at -80°C for subsequent determination of total protein concentration by Bradford Assay (Pierce Biotechnology) and IgM concentration by ELISA Bethyl Laboratoriess).
Lipoplexes were prepared in phosphate buffered solution (PBS), for subsequent determination of size and zeta potential by dynamic light scattering (DLS) and laser Doppler anemometry, respectively, using a Zetasizer Nanoseries ZS (Malvern Instruments) by diluting the sample 50x with dd-water.
Every two weeks, rats were individually housed in metabolic cages for 24 hr for subsequent determination of urine protein excretion using the benzethonium chloride method.
Supernatants were flash frozen in liquid nitrogen and stored at -80°C for subsequent determination of prostaglandin E2 (PGE2) content.
Segments of kidney were flash frozen in liquid nitrogen and stored at -80°C for subsequent determination of lipid contents and gene expression.
The supernatant was stored at −80°C for subsequent determination of eosinophil cationic protein (ECP), while the cellular pellet was resuspended in 0.3 mL of PBS.
Three hours after the start of mechanical ventilation, mice were injected with 100 μL of 14 mM 70-kD fluorescein isothyocyanate dextran (FITC-D; Invitrogen) in sterile water into the retro-orbital vascular sinus for subsequent determination of lung permeability.
Muscle biopsy specimens were either snap frozen then shipped for ETC determination in homogenates or muscle mitochondria were isolated from fresh muscle biopsy specimens with aliquots frozen for subsequent determination of ETC activities.
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