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Highly parasitic transformants were selected for subsequent assays.
The extracted DNA samples were stored at −20 °C for subsequent assays.
Finally, peroxidase-bound microparticles were washed with phosphate buffer three times for subsequent assays.
Among the analyzed compounds with the lowest free binding energy to the arginine kinase active site (<−6.96 kcal/mol), resveratrol was chosen for subsequent assays.
Two weeks after transfection, the cells were harvested and used for subsequent assays.
Based on the viability trend obtained, 50 µM was chosen for subsequent assays.
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This selection of 591 SNPs was available for subsequent assay design.
The remainder of the homogenate was centrifuged at 2500 g for 5 min, and the supernatant stored at −20°C for subsequent assay of cytokine concentrations.
The supernatants were stored at −80°C for subsequent assay.
Serum was separated and stored frozen at −80°C for subsequent assay.
The crude extract was centrifuged at 5433 × g for 15 min at 4 °C and used for subsequent assay.
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