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This selection of 591 SNPs was available for subsequent assay design.
The remainder of the homogenate was centrifuged at 2500 g for 5 min, and the supernatant stored at −20°C for subsequent assay of cytokine concentrations.
The supernatants were stored at −80°C for subsequent assay.
Serum was separated and stored frozen at −80°C for subsequent assay.
Arterial blood samples (5 ml) were collected from all patients into tubes containing citric acid and frozen for subsequent assay.
The crude extract was centrifuged at 5433 × g for 15 min at 4 °C and used for subsequent assay.
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Highly parasitic transformants were selected for subsequent assays.
The extracted DNA samples were stored at −20 °C for subsequent assays.
Finally, peroxidase-bound microparticles were washed with phosphate buffer three times for subsequent assays.
Among the analyzed compounds with the lowest free binding energy to the arginine kinase active site (<−6.96 kcal/mol), resveratrol was chosen for subsequent assays.
Plasma was collected and stored at −20°C for subsequent assays.
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