Sentence examples for for subsequent addition from inspiring English sources

We also propose that the first full-length "known" subunit splice variant should be named "v1", allowing for subsequent addition and standard nomenclature if additional variants are discovered in the future.

The cells were then counted using a hemocytometer and the appropriate number suspended in 50 μl DMEM for subsequent addition to the multiwell HSF cultures.

This short DNA duplex also contains numerous restriction sites including Xba I and Sal I for subsequent addition of mU6 and Kpn I and Pst I for future cloning of DNA insert encoding siRNA.

In addition to key roles in assembly, these longitudinal structural variations might provide the axial polarity and spatial specificity for subsequent addition of accessory structures, such as the subdistal and distal appendages, during basal body and centriole maturation.

The MTBE biodegradation rate increased three-fold from a mean of 6.6 ± 1.6 μg/L/day in uncontaminated aquifer microcosms for subsequent additions of MTBE, suggesting an increasing biodegradation capability, due to microbial cell growth and increased biomass after repeated exposure to MTBE.

To facilitate this conjugation an alteration of the molecular structure of the hapten was necessary to provide the appropriate functionality for subsequent additions.

Cells were first treated with tumor necrosis factor-α for 24 h, with subsequent addition of IMD-0354 for another 24 h and thereafter total RNA was extracted.

THP-1 cells were grown in suspension, then treated with PMA (320 nM/1 × 10 cells) for 6 h with subsequent addition of IL-4 (20 ng/ml) for 18 h (total of 24 h).

Astrocytes (ASs), comprising >98% purity, were generated by dissociation of secondary NSC clones using 0.05% trypsin for 15 minutes at 37°C and re-propagation of individual cells in epidermal growth factor (EGF) for 7 DIV with subsequent addition of bone morphogenetic protein 2 (BMP2) for 5 DIV [99], [100].

Astrocytes (ASs) were obtained by dissociation of NSC clones using 0.05% trypsin (Sigma) for 15 minutes at 37°C and re-propagation of individual cells in epidermal growth factor (EGF) for 7 DIV with subsequent addition of bone morphogenetic protein 2 (BMP2) for 5 DIV [97], [98].

Reductive amination was performed by the addition of 20 µL 2-aminopyridine solution to the treated fractions followed by heating at 90°C for 1 h, and the subsequent addition of 20 µL borane-dimethylamine complex solution with further heating of the mixtures at 80°C for 1 h.