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Based on these data, we cloned the 675 bp fragment containing the functional urg1 promoter (Purg1) into several PCR-based targeting vectors for straightforward integration of the promoter upstream of selected genes in their normal chromosomal locations (Figure 3).
Together, they allow for straightforward parallelization.
It calls for straightforward collaboration between officials.
Bowie was never one for straightforward autobiography.
The proofs are omitted for straightforward results.
5) The electroporation figure could do with some straightforward population analysis, for example location of electroporated cells superimposed on a normalized barrel.
The location of this second copy was not revealed in the present study but it should be straightforward to search for its location using next-generation sequence data in particular since the transposed fragment is only about 2 kb in size.
But despite this guidance, the task of locating bodies was far from straightforward, as the locations were originally chosen for their remoteness to minimise discovery of the graves.
For starters, location, location, location.
As for the location?
Search for a location.
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