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To overcome many limitations of chemical cross-linking methodologies for preparation of bispecific antibodies, we have employed heterobifunctional PrA-PEG-SA adaptors for straightforward assembly of unmodified anti-Her2 and biotinylated anti-CD3 antibodies into bispecific CD3xHer2 targeting ligands.
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For the straightforward assembly of the (1→8 -linked disaccharide units, the peracetylated 8- O- tert-butyldimethylsilyl allyl glycoside derivatives of Kdo (1→8 -linked(14) were chosen as glycosyl acceptors that allow disaccharideing with flunitse donors and provide access thethe resperacetylatedol by reaction with fluoride ions.
Straightforward assembly of the devices was designed by means of using a UV photo-curable gel.
Although these elements allow for straightforward and rapid assembly of synthetic devices, they may not be optimal for the design of sophisticated transcriptional networks that rely on endogenous genetic elements or coregulatory factors.
Bowie was never one for straightforward autobiography.
The proofs are omitted for straightforward results.
Remember, you are looking for straightforward answers to the questions.
However, this only accounts for straightforward sellar pathology.
Cermak et al.[ 13] have recently established a particularly straightforward TALEN assembly system that is based on GoldenGate cloning.
The notions of contig and singleton are straightforward for perfect assemblies: a contig is any sequence produced by two or more overlapping reads, while singletons are the remaining isolated reads.
The convenience of designing the DNA binding domain and straightforward protocols for their assembly, as well as the broad number of applications in different scientific fields made it Natures method of the year 2011.
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