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In Figure 2, I suggest a workflow for stepwise development of tools in a given parasitic nematode, starting with basic transgenesis methods and FP reporters, and building to sophisticated genome editing tools.
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The RIP1-Tag2 mouse is an orthotopic model of multistage carcinogenesis and is considered to be a more accurate model for the stepwise development of tumors than conventional subcutaneous models with injected tumor cells.
Although IM is a time-consuming process, it provides a useful protocol for the stepwise development of interventions and the planning of the implementation and evaluation of intervention programmes.
The model is developed by a series of refinements following the stepwise development approach.
In this paper, the stepwise development of a novel multivalent high-affinity ligand for CD22 is described.
TECs, composed of cortical and medullary TECs, are derived from a common bipotent progenitor and undergo a stepwise development controlled by multiple levels of signals to be functionally mature for supporting thymocyte development.
Industrial upgrading (IU), the stepwise development of manufacturing skills from simple to more complicated tasks, can be a highly effective approach for late entrants.
It contains rather specific provisions on the stepwise development and testing of agricultural GMOs.
The stepwise development of eradication methods in a series of offshore New Zealand islands is remarkable in its tenacity, ingenuity and impact.
We observed a stepwise development of the response to TLR4 and TLR9 stimulation during this period.
These results suggested that these miRNAs might play important roles in the stepwise development of NPC.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com