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Taken together, our hybrid-spheroids can be an ideal approach for stem cell expansion and serve as a potential carrier for bone regeneration.
Overall, this study provided a new and valid strategy for stem cell expansion and osteo-differentiation in 2D or 3D culture systems, possessing potential applications in 3D bio-printing and tissue regeneration.
We proposed an alternative method of stem cell culture that focuses on the use of Zn++ Finger nanoparticles for stem cell expansion and transdifferentiation modulation in vitro and in in vivo spinal cord injury models.
Three-dimensional culture conditions can therefore provide an ex vivo mimicry of bone marrow, recapitulate the desired niche, and provide a suitable environment for stem cell expansion and differentiation.
The knowledge of the role of NO in the Hypoxia Response and the mechanism that helps to maintain self-renewal in pluripotent cells in normoxia, can help to the design of culture media where NO could be optimal for stem cell expansion in the performance of future cell therapies.
Accordingly, to maintain self-renewal ability of stem cells, a stromal-free suspension culture has been rapidly adopted as an alternative to stromal layer culture for stem cell expansion due to their chemically defined nature.
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Thus, it is indicative of stem cell expansion.
Methodologies that are able to functionally measure symmetric cancer stem cell (LTP) division under a variety of experimental conditions will benefit the targeting of this component, which is important for cancer stem cell expansion.
23, 24 Interestingly, PGE-2 was shown to stimulate hematopoietic stem cells 25 and EP4 activation was reported to be essential for hematopoietic stem cell expansion.
DNA methylation patterns can also act as markers for tracing stem cell expansion and tumor growth (Kim et al., 2005; Shibata and Tavaré, 2006; Yatabe et al., 2001).
In breast cancer, fibroblast-derived POSTN is crucial for breast cancer stem cell expansion during metastatic colonization in lung (Malanchi et al., 2012; Wang and Ouyang, 2012), which is very different from the function of GSC-derived POSTN in GBMs.
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