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The sub-analysis in females did not show any difference from the overall analysis, and the male sample was too small for statistical calculation.
For statistical calculation the independent variables were coded as follows: {x}_i = left({X}_i-{X}_{mathrm{o}}right)/delta {X}_i (1).
We transformed the numbers of true positive, false positive, false negative, and true negative based on the provided indices of sensitivity, specificity, and sample size values for statistical calculation.
For statistical calculation, the experimental variables X i have been coded as x i according to the following transformation equation: x_{i} = frac{{X_{i} - X_{0} }}{delta X} (2 where x i is the dimensionless coded value of the variable X i, X0 is the value of x i at the center point, and ( delta X )is the step change.
Although the sample size (comprising only 11 tumor samples) used for quantitative real-time PCR analysis was not sufficient for statistical calculation, the qPCR results did show alterations in gene expression levels.
Excel was used for statistical calculation.
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Microsoft Office Excel 2007 and PASW statistics (SPSS) 18.0 were used for statistical calculations.
The Statistical Package for the Social Sciences (SPSS) version 16.0 was employed for statistical calculations.
For statistical calculations, the variables X i are coded as x i.
Graph Pad Prism 5 was used for statistical calculations (Graph pad Software, San Diego California, USA).
The R-software (version 3.1.3, R Foundation for Statistical Computing, Vienna, Austria) was used for statistical calculations.
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