Sentence examples for for staining development from inspiring English sources
Diaminobenzidine was used for staining development and the sections were counterstained with haematoxylin.
Gels were stained with a 0.1% silver nitrate solution containing 0.5% formaldehyde for 30 min after gel fixation on 10% acetic acid solution for 20 min. Staining development used a 6% sodium carbonate solution with 0.5% formaldehyde and 2µg of sodium thiosulphate for 3 min and the reaction was stopped with 10% acetic acid solution.
After time-sensitive stain development, sections were counterstained in hematoxylin (Sigma) for identification of cell nuclei.
For double staining, colour development with 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium was in addition applied (yielding a deep blue colour) as previously described (Jørgensen et al, 2009).
Upon fertilization, Ce-GnRHR staining becomes diffuse throughout the developing egg, although staining appears to increase during egg development (see Figs. 3B for staining of laid eggs).
Leaves were harvested for staining 6 or 7 days after inoculation depending on the development of the fungal infection.
Freud also developed a new method for staining nervous tissue.
"Always checking behind me, to check for staining".
Score for staining intensity: 0 = negative, 1 = weak staining, 2 = moderate staining, 3 = strong staining.
The beads were washed four times in IP buffer, once in distilled water, eluted with SDS-PAGE sample buffer, and analyzed on 12% Tris-glycine gels, followed by blotting, staining, and development as for immunoblots.
