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The BestKeeper software [ 52] was used to search for stable reference genes among all genes tested.
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In this regard, there are unmet needs to identify and validate a set of stable reference markers for data standardization in human liver tissues.
An important factor for normalization is the use of stable reference genes.
For further characterization and identification of stable reference genes, we performed a geNorm pilot study [ 51] and calculated the coefficient of variation (CV) for all 20 potential reference genes.
The developed relative displacement sensor is an innovative design offering some advantages and unique features, and is a much easier and economical method for structural health monitoring due to the simplicity of its direct measurement of relative displacement without the requirement for a stable reference point.
For this, first the normalisation factors (NF) were calculated for the most stable reference genes (with lowest M-value) and then for other genes by stepwise inclusion of the reference gene that remains most stable.
For example, the most stable reference gene for Talaromyces marneffei was GAPDH, followed by TUBA, and ACTB (Dankai et al. 2015).
For the purpose of obtaining a stable reference gene for optimal normalization of RT-quantitative PCR gene expression assays, we tested four candidate reference genes.
NormFinder is another Excel application, which uses a model-based approach for identifying the most stable reference genes, based on combining samples into groups.
RefSeq is intended to be a comprehensive and non-redundant set of protein sequences that provides a stable reference for genome annotation.
This use of eye tracking is easier than using the eyes as a precision pointing device, which is difficult because the eyes constantly explore the environment and do not offer a stable reference for a screen pointer.
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