Exact(3)
For spreading analysis, cells were imaged immediately after plating with 20 frames/h for 3 h using 20 objective and Axiocam MRm camera (Carl Zeiss).
For spreading analysis, cells were fixed overnight in 4% paraformaldehyde and blocked with 0.1% v/v Triton X-100 in PBS for 2 min at room temperature.
For spreading analysis, cells were trypsinized, resuspended in complete medium, plated on sterile coverslips coated with an adhesive substrate, and allowed to spread for various times.
Similar(57)
For cell spreading analysis, 3×105 cells were plated on gels on coverslips in growth media and allowed to adhere and spread for 20 hours at 37°C.
In spreading analysis, cells were pretreated for 30 min with latrunculin B, or for 1 h with nocodazole.
The left epididymis and testicle were removed and weighed, and the cauda epididymis was used for sperm analysis; the left testicle was used for a meiotic chromosomal spread analysis and alkaline comet assay.
For meiotic nuclear surface spread analysis, spermatocytes or prophase I oocytes were prepared using the dry-down method as previously described (Peters et al, 1997; Kolas et al, 2005).
He responded, "Thanks for spreading the word".
Use refrigerated portion for spreading between layers.
Use the inside portion for spreading.
Samples were analyzed with flow cytometry to determine cell cycle distribution, by Western blot to determine p53 and ID1 expression, and for mitotic spread analysis to determine mitotic index.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com