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Progress in biomarker research has been frustratingly slow due to the poor clinical trial design and the lack of standards for specimen collection, biomarker analysis, and data reporting.
A dry 'paper smear' method for specimen collection and a simple single tube protocol was employed for PCR detection of HPV infection.
All disease related biobanks at Mayo Clinic and the Community Biobank (Olson et al. 2013) have standardized all procedures for specimen collection, DNA extraction, serum/plasma processing, and storage to enable case vs control analyses that are matched for specimen quality control.
All controls for specimen collection and processing were appropriately negative for HPV and β-globin.
We are grateful to Chris Burger, Mariana Bulgarella and John M. Pearce for specimen collection.
Plasticware used for specimen collection, processing and analysis was acid soaked and rinsed with distilled water prior to use.
Monika Andersson and the staff at the Department of Neurosurgery, Lund University Hospital are thanked for specimen collection and patient information.
The acceptability of blood, urine and vaginal swabs for specimen collection was also assessed, and standardized methods of collection were developed.
AM was responsible for specimen collection.
EORTC is developing adopted standardized protocols for specimen collection, storage, and processing.
NA and MAH were responsible for specimen collection, data analysis, summarization of results and manuscript preparation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com