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Anodic current was further continued for specified times.
Reactions were incubated at 22°C for specified times, and then terminated by the addition of loading buffer, denatured and separated by electrophoresis in 7 M urea-17% polyacrylamide gels.
For the soil-grown plant drought tolerance test, one-week-old seedlings were transplanted to the soil for two weeks under standard growth conditions, and then plants were subjected to progressive drought by withholding water for specified times.
Cells were then treated with medium supplemented with 2 or 5 μ mitomycin C for specified times.
The next day, medium was removed and PRF medium supplemented with the intended treatments and growth factors was added and incubated for specified times.
Total RNA was extracted from cells using TRIzol reagent (Applied Biosystems, Life Technologies), following the manufacturer's instructions, after treatment for specified times with 27-OH or HNE.
Similar(48)
The buffer was artificially contaminated ("spiked") with mixed aflatoxin standard and heated for specified time periods.
Work exceptionally long hours when you need to or want to, but do so consciously, for specified time periods, and to achieve specific goals.
The growth rate (by measuring thickness for specified time process), refractive index and stress of the deposited SiO2 films have been determined by ellipsometry technique.
If this sounds familiar, you should try to work exceptionally long hours when you need to or want to — but do it consciously, for specified time periods, and to achieve specific goals.
Cox and Weibull proportional hazards models for survival time (t SURV) were estimated, with TVC assumed to have constant effects for specified time intervals following hospital arrival.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com