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All antibodies were examined for specificity prior to use.
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This result and the minor expression patterns observed in MSP1 seedlings highlights the need for experimental verification of specificity prior to further practical analyses.
Because some genes are recognized by more than a single probe set, each of which is characterized by an individual specificity and sensitivity that uniquely contribute to the gene expression value, a mean gene expression value was calculated after weighting each probe set for its own sensitivity and specificity, prior to the analysis.
The prior distribution of the sensitivity of the commercial ELISA used to test the serum samples for the presence of Yersinia antibodies was beta 63,29), and the specificity prior distribution was Sp ~ beta 6.0,1.1).
eRefers to specificity prior to filtering.
All real-time primer sequences were validated for specificity and efficiency prior to use.
informative priors for specificities alone: the prior density of the specificities alone was centered at their true values, whereas the sensitivities and prevalence were assigned as non-informative priors.
We used 2 informative priors for the sensitivity and specificity of T1 (prior distribution ranging 0.7 ± 0.1 for sensitivity and 0.9 ± 0.05 for specificity) and uniform priors for the other parameters (i.e., sensitivity and specificity of T2 and prevalence).
Test sensitivity, specificity and the true prevalence were specified as Beta probability distributions with prior distribution parameters of 7 and 4 (mode 67) for sensitivity, 10 and 2.5 (mode 86) for specificity and a non-informative prior for the true prevalence.
For both ELISA tests a uniform prior distribution for specificity Be (0, 1) was assumed.
All primers were confirmed for specificity via cloning and sequencing prior to use (data not shown).
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