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For specificity, control blocking experiments were run with unlabelled specific double-stranded oligonucleotides or a respective mutant variant (5' -CatCAGAGGCGACTTTCCGAGGGATG-3') at 10-fold and 100-fold molar excess of the labelled NF-κB probe.
For specificity control, a sense probe was used.
The corresponding isotype control antibody (R&D Systems) was applied for specificity control.
Anti-isotype antibodies (BD Biosciences) were used in parallel, for specificity control.
After completion of PCR, the melting curves for specificity control were recorded.
Omission of primary or secondary antibodies or both was performed for specificity control.
Similar(51)
In other words, in the absence of Rab3A, fusion remained sensitive to anti-NSF antibodies (please see [ 8] for specificity controls).
For specificity controls, aliquots of three antisera containing antibodies reactive with heterologous flaviviruses (tick-borne encephalitis-, yellow fever- and Dengue virus) provided by reference laboratories of our network and one serum with a known unspecific reactivity against cell and mitochondrial structures provided by EUROIMMUN AG, Lübeck, Germany were included.
For antibody specificity control and adjustment of auto-fluorescence, staining excluding the primary antibodies was included in every experiment.
In addition, specificity control for the staining was included using cells treated with 500 nmol/L H2O2 solution.
Laminin coating was used as matrix specificity control for differentiation.
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