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Some genes appeared unique for specific time points.
Asterisks indicate statistically significant differences between the PCP and saline groups for specific time points (*p<0.05 and **p<0.01; Newman Keuls post hoc test).
Cells were incubated for specific time points (5, 15, 30, 60 and 120 min, respectively) at 37°C; non-specific binding was determined by adding an additional 100 μL of natGa-labelled tracer to a final concentration of 1 μM of the corresponding peptide.
A sliding windowing procedure was adopted that allowed discrete spectra to be calculated for specific time points across the entire data segment.
The cells were starved for two days, then left unstimulated, or simulated with 50 ng/ml PDGF-BB (Sigma-Aldrich, Saint Louis, MO) in six well plates in serum-free medium for specific time points.
We extracted data for specific time points before and after the intervention.
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The free choice of specific time points for pulse labeling of the receptor and the sequential labeling of receptor subpopulations with spectrally distinguishable fluorophores provide a promising tool for the imaging of consecutively expressed GPCRs and their spatiotemporal organization [ 160].
The spectra for three specific time points, 0, 48 and 240 h, are shown in Figure 3.
Generic mortality scoring systems for children admitted to intensive care units (ICUs) have been developed for use at specific time points in the ICU stay.
Event-related endpoints were analyzed using the Kaplan-Meier method, providing 95% confidence intervals (CIs) for proportions at specific time points.
For comparisons involving specific time points, we identified the set of strains that exhibited significant fitness defects (relative to the first time point) in the flight condition but not in the ground condition.
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