Sentence examples for for specific target loci from inspiring English sources
To obtain transgenic crops whose genome is identical to that of the host in every respect except for specific target loci, multiple backcrosses will be necessary.
This being the first reported physical map of the flax genome, it provides a frame work for accessing specific target regions harbouring loci with economic/biological importance for marker development and positional cloning using large insert BAC clones.
Allele exchange cassettes specific to target loci were either constructed by SOE PCR [ 45] or commercially synthesised.
The alternative of making barcoded allele specific primers for each target locus would be cost-prohibitive.
The remaining library was enriched for target loci and was re-amplified to incorporate sample specific indices.
Low coverage for target loci was extremely rare, other than for the excluded locus (see Methods).
In another example, we demonstrated focal immobilization and hence, potential for targeting specific loci with the IT6-A7 partidentifiedtifrom from the PHD12 library, with the targeting sequence SQIDYATGPRQA displayed on the phage.
WT primers produce a 650 bp band and primers specific for the targeted locus produce a 340 bp band using the PCR conditions: for denaturing, 94°C for 30 seconds; for annealing, 62°C for 60 seconds; for extension, 72°C for 60 seconds for 34 cycles.
All of the non-insertion-deletion mutations conserved the open reading frame for ARF, suggesting specific targeting of the p16 locus for inactivation, a phenomenon reported in other tumor types as well [34].
