It seems likely that these findings result from cell-specific roles for Rab25 in the trafficking of specific cargoes.
Sec24 is responsible for specific cargo recognition and binding.
The FERM domain of MYO10 is reportedly a binding domain for specific cargo molecules, including integrins (Zhang et al, 2004), Mena/VASP (Tokuo and Ikebe, 2004), DCC (Zhu et al, 2007), and VE-cadherin (Almagro et al, 2010).
Sorting signals are required to target specific cargoes to the right destinations, although the signals and machinery needed for cargo sorting are not entirely understood.
The Importin α1 protein shows a tandem array of ten armadillo (ARM) repeats, where the ARM domains 1 to 4 (major site) and the domains 4 to 8 (minor site) are responsible for recognition and binding of specific cargoes [ 47, 48].
Collectively, these experiments indicate that SFT2D2 and ZDHHC5 are important for recycling of specific cargo proteins to the Golgi, whereas loss of GRINA may have more wide-ranging effects on TGN structure and stability of a number of membrane proteins.
These interactions may indicate a requirement for the transport of specific cargo(s) in the glc7-E101Q muthat tharearequiredred for viability.
It seems likely, therefore, that each paralog mediates the inclusion of specific cargo proteins into vesicles for subsequent export from the ER.
GRASP55 is especially known for its role in maintaining Golgi architecture, but also plays a role in Golgi transport of specific cargo proteins bearing a C-terminal valine residue.
Directional transport of specific cargos is tuned to specific molecular motors and specific cytoskeletal tracks.
